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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
02/03/2017 |
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Actualizado : |
07/03/2017 |
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Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
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Idioma : |
Inglés |
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Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02512naa a2200217 a 4500
001 1056750
005 2017-03-07
008 2015 bl uuuu u00u1 u #d
100 1 $aGARCIA, J.P
245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource]
260 $c2015
520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease.
650 $aENFERMEDADES DE LOS ANIMALES
700 1 $aGIANNITTI, F.
700 1 $aFINNIE, J.W.
700 1 $aMANAVIS, J. .
700 1 $aBEINGESSER, J.
700 1 $aADAMS ,V.
700 1 $aROOD, J.I.
700 1 $aUZAL, F.A.
773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
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| 28. |  | SYVERTSEN, J.P.; GOÑI, C.; OTERO, A. Fruit load and canopy shading affect leaf characteristics and net gas exchange of 'Spring' navel orange trees. Tree Physiology, September 2003, Volume 23, Issue 13, Pages 899-906. OPEN ACCESS. Article history: Received December 11, 2002 / Accepted March 8, 2003 / Published online August 1, 2003.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
| Biblioteca(s): INIA Las Brujas. |
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| 29. | | OTERO, A.; GOÑI, C.; JIFON, J.L.; SYVERTSEN, J.P. High temperature effects on citrus orange leaf gas exchange, flowering, fruit quality and yield. (Conference paper). Acta Horticulturae, 2011, no.903, p.1069-1074. (Acta Horticulturae; 903). ISHS Acta Horticulturae 903: IX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems. Editor: T.L. Robinson.
Publication date: 1 August 2011| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 2 |
| Biblioteca(s): INIA Las Brujas. |
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| 32. | | RIVAS, F.; BERNAL, R.; BERTALMIO, A.; BUENAHORA, J.; GOÑI, C.; LADO, J.; PEREZ, E.; OTERO, A.; MARA, H. La citricultura bajo estrés: un enfoque integral. Revista INIA, 2009, no. 17, p. 68-72.| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó; INIA Treinta y Tres. |
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| 35. | | OTERO, A.; GOÑI, C. Manejo del suelo en cítricos: algunos efectos del uso de mulches In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Citrícola. Reduciendo incertidumbres: el riego en la productividad de los cítricos. Avances de investigación. Salto Grande, Salto (Uruguay): INIA, 2009. p. 54-59 (INIA Serie Actividades de Difusión ; 576)| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 37. | | BENNADJI, Z.; ALONSO, R.; CASTRO, M.; CIGANDA, V.; GOÑI, C.; PÉREZ DE VIDA, F.; VÁZQUEZ, D.; BERGER, A.; GASO, D.; CERETTA, S.; FASSIO, A.; SALDAIN, N.E.; OTERO, A. Mejoramiento genético más ecofisiología: generando espacios interdisciplinarios para la disminución de la vulnerabilidad al cambio climático Revista INIA Uruguay, 2011, no. 25 p. 43-48 (Revista INIA; 25)| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 38. | | GOÑI, C.; OTERO, A. Momentos de riego en Satsuma Owari, Spring Navel y Ellendale In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Citrícola. Reduciendo incertidumbres: el riego en la productividad de los cítricos. Avances de investigación. Salto Grande, Salto (Uruguay): INIA, 2009. p. 28-48 (INIA Serie Actividades de Difusión ; 576)| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 39. | | GOÑI, C. Muestreo foliar en mandarinas y naranjas para el área norte de Uruguay. ln: Congreso Nacional de Horticultura, 8.; Seminario Regional de Frutilla, 2001, Salto, Uruguay. Resúmenes. Montevideo (Uruguay): SUH; INIA, 2001. p. 60 "Sociedad Uruguaya de Horticultura; Instituto Nacional de Investigación Agropecuaria, Uruguay"| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas; INIA Salto Grande; INIA Tacuarembó; INIA Treinta y Tres. |
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| Registros recuperados : 49 | |
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